A study conducted by Eunjoo KIM assessed skin changes in 32 individuals in their forties who had their sleep limited to four hours per night for six nights. Several parameters, including elasticity, crow's feet wrinkles, and frown lines were measured. The parameter for analysing elasticity was R2, calculated using the following formula: R2 = Ua/Uf, where Uf is the maximum skin distension and Ua is the total skin distension. The closer the R2 value is to 1, the more elastic the skin is. The wrinkles at the corner of the eye and the frown line were measured based on their average depth (in mm).
The results show that skin elasticity gradually decreased after sleep reduction, moving from an R2 of 0.65 to 0.59 with a noticeable reduction between the fifth and sixth day. Moreover, after just one day of sleep deprivation, crow's feet and frown lines significantly increased by about 20 μm to 21.5 μm in depth.
Other studies have shown an association between lack of sleep and an increase in pro-inflammatory cytokines such as TNF-α. The latter binds to specific receptors on the surface of skin cells, thereby triggering intracellular signalling pathways, such as the NF-kB pathway. Indeed, the activation of these pathways leads to the expression of genes involved in inflammation, including those associated with the production of reactive oxygen species (ROS).
It can also increase the activity of certain enzymes, such as NADPH oxidase and xanthine oxidase, which are involved in the production of ROS. ROS are free radicals known to cause oxidative stress, and are supposedly involved in skin ageing through the destruction of collagen and the elastin network, responsible for the elasticity and integrity of the skin. Therefore, less sleep can accelerate skin ageing.