How is Vitamin E obtained?

Today, the majority of the vitamin E found in cosmetic products is extracted from vegetable oils. For this process, one or more oils can be used. Most commonly, these are soybean oil and/or sunflower oil, which are very rich in tocopherols. The extraction is carried out using an organic solvent, in which the non-saponifiable elements of vegetable oils such as vitamin E are soluble, while the fatty acids, glycerol and other hydrophilic compounds remain in the aqueous alkaline phase. The most frequently used solvent is hexane, either alone or with a small fraction of a more polar solvent such as ethanol, ethyl acetate or diisopropyl ether. It is also possible to use acetone, diethyl ether or a 2:1 mixture of chloroform-methanol. In the latter case, this is referred to as Folch extraction.

The extraction of vitamin E from vegetable oils is typically carried out using a vortex or equivalent stirring systems . These devices allow for vigorous yet controlled agitation of the solution, which reduces the extraction time. The solvent is then evaporated using a rotary evaporator under reduced pressure. The temperature depends on the solvent used but is often within ranges between 40 and 60°C. Following this, the extracted vitamin E undergoes various purification processes to remove impurities and obtain a purer molecule. Distillation, filtration, and decantation stages are notably involved. Throughout the process, the quality of the vitamin E is tested by several chromatography methods (HPLC, TLC, CEC...).

It is also possible to synthesise vitamin E in a laboratory. However, this method is being used less and less due to its cost and technical complexity. Here are the main steps of this chemical synthesis, noting however that these may slightly vary from one laboratory to another.

1. Formation of the chromanol ring.

   The initial stage of alpha-tocopherol synthesis involves hydroquinone, an aromatic compound also known as 1,4-benzenediol, and isophytol, a natural precursor of tocopherols derived from phytol, a component of plant chlorophyll. These two molecules react together to form a chromanol ring, the fundamental structure of vitamin E.

2. Alkylation of the chromanol ring.

   Once formed, the chromanol ring must be alkylated to introduce the isoprenoid side chains. Among the intermediates commonly used to carry out this reaction, we can mention geranylgeranyl pyrophosphate.

3. Hydroxylation and Methylation.

   In conclusion, successive hydroxylation/methylation reactions are carried out to establish the alcohol group of alpha-tocopherol as well as its carbon chain.

4. Purification and Isolation.

   Once the reactions are completed, alpha-tocopherol is purified and isolated using chromatography techniques such as high-performance liquid chromatography, thin-layer chromatography, or size-exclusion chromatography. The aim is to obtain a product that is as pure as possible, free from reaction by-products.